Application

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected RIM 2 in human brain tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected RIM 2 in human brain cells.

Research CategoryNeuroscience

Anti-RIM 2 Antibody is an antibody against RIM 2 for use in western blotting, IHC & immunofluorescence.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. RIM 1 and RIM 2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like RIM 1, RIM 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner.

Immunogen

KLH-conjugated synthetic peptide corresponding to the center region of human RIM 2.

Epitope: center region

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat brain tissue lysate with and without blocking peptide.

Western Blotting Analysis: 1 µg/mL of this antibody detected RIM 2 in rat brain tissue lysate with and without blocking peptide.

Specificity

This antibody may recognize many isoforms and is predicted to have no cross-reactivity to other RIM proteins.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~86 kDa observed. Uniprot describes 3 isoforms produced by alternative splicing ranging between ~31 kDa and ~160 kDa